Plasma lipids and XbaI polymorphism on APOB-100 gene in a group of children and their parents

Abstract

Introduction: Some studies have shown association between XbaI polymorphism of apolipoprotein B (apoB) gene and serum levels of lipids and apolipoprotein B, but not in others. Objective: to determine the relationship between plasma lipids, apoB, XbaI polymorphism in a group of boys and girls between 4 and 18 years and their parents. Methods: We studied 63 children and 85 parents. The children were divided into two groups: children with hypercholesterolemia (CHC), total cholesterol (TC)>175 mg/dl and children without hypercholesterolemia (SHC), TC<175 mg/dl, were determined: XbaI genotype (for the polymerase chain reaction (PCR) and digestion with XbaI restriction enzyme), lipid profile, ApoB and apoA1. Results: Individuals were X1X1 genotype, 28.37%, 64.86% X1X2, and 6.75% X2X2. The allele frequency was 0.61 (X1) and 0.40 (X2). X1X1 genotype frequency was 0.39 and 0.28 children CHC and SHC respectively and 0.30 and 0.19 in parents respectively. There were no differences in total cholesterol and LDL-C, between X1 and X2 alleles in each group. In the group of parents and children CHC, the X2 allele showed levels of triglycerides (TG), higher, although not was significant. Conclusions: Allele frequencies and gene were similar to those described for Western populations and different from those reported for the eastern population. In this population, no relationship was found between the polymorphism and levels of lipids and apoB, however, additional studies are needed.